Hybridoma Sequencing Service
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| Our Services |
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Hybridoma sequencing protects valuable antibodies from mutation or loss, and enab les antibody e ngineering and recombinant expression. We have sequenced 500+ hybridomas for customers. We can not only provide the antibody sequencing service, but also the recombinant antibody expression service after sequencing to ensure the accuracy.
Our advantages:
- Fast, complete, accurate and highly reliable sequencing results to ensure the confidentiality
- Other recombinant antibody related services, including recombinant antibody expression, antibody humanization, etc
- The sequencing service is applicable for hybridoma cell lines from most rodent species and antibody isotypes.
- Highly versatile sequencing services to reveal both variable region and full-length antibody protein sequences.
- One-stop service from mRNA extraction to sequence determination as well as antibody format conversion.
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Material
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Specification |
Timeline |
Deliverables |
Pricing |
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1. hybridoma cell
2. subtype and source
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• RNA extraction
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1 day
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• V-region sequence of heavy and light chain
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Quote
(086)027-65522449
(086)027-59325199
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• rt-PCR
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1 day |
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• PCR amplification
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1 day |
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• Sequencing
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1 week |
| Vector Construction (Optional) |
1 week |
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Note:
trizol-treated cells are easier to ship. The protocol is：
Blow the cultured cells with the medium, the number of cells is more than 1×106 . and the cells should be in good growth condition.
The cells were harvested, centrifuged, and the supernatant was discarded. According to the amount of cells, 1-2mL Trizol was added (1×106 plus 1mL, 5×106-1×107 plus 2ml,1×108 plus 3mL), and stored at -80℃，
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VH and VH Sequencing Result
Protein Expression Services
