Stable Cell Line Construction|Stable Cell Line|Stable cell Service

Stable Cell Construction

Our Services

Stable cell lines are widely used in the production of recombinant proteins and antibodies, detection and analysis, gene editing, functional research et al. Our stable cell line construction service can effectively improve the protein production capacity. We integrate all experimental steps and provide one-stop services from gene sequence to stable strain delivery.

Our related services include:

Gene design and codon optimization
Construction of multiple expression vectors
Transient transfection and detection
Construction and detection of stable transformation cell line
Expression in large scale

Service Details (Stable HEK293 Cell Line Construction)

Steps	Specification	Timeline	Deliverables	Pricing
Gene synthesis	• Gene synthesis and codon optimization	1 week	•Expression Vector	Quote (086)027-65522449 (086)027-59325199
Vector construction	• clone cDNA into expression vector • plasmid sequencing • large-scale plasmid preparation	1 week	•Expression Vector
Expression evaluation	• transfection into proper host cell（HEK293) • small-scale transfection •SDS-PAGE analysis to evaluate protein expression	1 week	• Expression evaluation result
Transient transfection and purification（30ml）	• transfection into suspension cultured cells • protein purification • SDS-PAGE and ELISA analysis	2 weeks	• Feasibility report • If expression is feasible, provide a certain amount of protein sample to the client based on the expression.
Stable cell line construction	• stable transfect and screen • identification（qPCR & FACS） • Subcloning • identification（qPCR & FACS） • stable cell line pool	10 weeks	• 2-3 stable cell line • report
Large-scale expression and purification (optional)	• 1L Flask or fermentation cylinder culture • Affinity Purificaiton • SDS-PAGE analysis	Depend on scale	• Purified protein • SDS-PAGE report • CoA

Service Details (Stable CHO Cell Line Construction)

Steps	Specification	Timeline	Deliverables	Pricing
Gene synthesis	• Gene synthesis and codon optimization	1 week	•Expression Vector	Quote (086)027-65522449 (086)027-59325199
Vector construction	• clone cDNA into expression vector • plasmid sequencing • large-scale plasmid preparation	1 week	•Expression Vector
Expression evaluation	• transfection into proper host cell（CHO) • small-scale transfection •SDS-PAGE analysis to evaluate protein expression	1 week	• Expression evaluation result
Transient transfection and purification（30ml）	• transfection into suspension cultured cells • protein purification • SDS-PAGE and ELISA analysis	2 weeks	• Feasibility report • If expression is feasible, provide a certain amount of protein sample to the client based on the expression.
Stable cell line construction	• stable transfect and screen • identification（qPCR & FACS） • Subcloning • identification（qPCR & FACS） • stable cell line pool	10 weeks	• 2-3 stable cell line • report
Large-scale expression and purification (optional)	• 1L Flask or fermentation cylinder culture • Affinity Purificaiton • SDS-PAGE analysis	Depend on scale	• Purified protein • SDS-PAGE report

Our Promise

• The high-efficiency expression vector makes the protein more stable and with higher activity

• High-density suspended cells used as expression host

• From sequence optimization, transient transfection, to protein / antibody purification and data sorting, only 4 weeks

Relative Services

Custom Services

Protein Expression Services

Custom mAb Services

Custom pAb Services

Peptide Services

Stable Cell Construction