Recombinant antibody is a monoclonal antibody expressed in vitro by gene engineering. Recombinant antibody technology makes it possible to prepare humanized antibody. And it also realize mass and stable production of monoclonal antibody without immunization. With rich experience of recombinant antibodies, Dia-an provide you with various forms of recombinant antibody services, including antibody sequencing, recombinant antibodies expression, chimeric antibody expression, scFv, Fab, single domain antibody (sdab) and diversified Fc fusion protein expression.
Our advantages:
● n o immunization is required, avoiding the human immune response caused by mouse monoclonal antibody
● good consistency, good repeatability of prepared antibody and small difference between batches
● recombinant transformation can be carried out, including antibody fragment expression, homotypic replacement, chimeric antibody, antibody humanization, etc
● mass preparation of antibodies: stable transfection and screening of stable cell lines are suitable for rapid industrialization of monoclonal antibody drugs
Steps
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Specification |
Timeline |
Deliverables |
Pricing |
Gene synthesis |
• Gene synthesis and codon optimization
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1 week
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•Expression Vector
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Quote
(086)027-65522449
(086)027-59325199
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Vector construction |
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
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1 week
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Expression evaluation |
• transfection into proper host cell(HEK293/CHO)
• small-scale transfection
•SDS-PAGE analysis to evaluate protein expression
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1 week
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• Expression evaluation result
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Transient transfection and purification(30ml)
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• transfection into suspension cultured cells
• protein purification
• SDS-PAGE and UV analysis
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2 weeks
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• Feasibility report
• If expression is feasible, provide a certain amount of protein sample to the client based on the expression. |
Large-scale expression and purification (optional) |
• 1L Flask or fermentation cylinder culture
• Affinity Purificaiton
• SDS-PAGE analysis
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Depend on scale
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• Purified protein
• SDS-PAGE report
• CoA |
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