Steps
|
Specification |
Timeline |
Deliverables |
Pricing |
Immunogen Preparation
|
• Recombinant protein expression
• Peptide synthesis and conjugation
|
1-4 weeks
|
• Design report
• Protein sample
• SDS-PAGE result
|
Quote
(086)027-65522449
(086)027-59325199
|
Immunization and Titer test |
• 4-8 rats immunization
• Anti-serum titer ELISA test
|
8-10 weeks
|
• Anti-serum sample
• ELISA titer
|
Fusion & Screening |
• Hybridoma fusion
• Positive clones screening
• 2 rounds of subcloning
|
4-6 week
|
• 1-3 ELISA positive clones
• 1-5 ml supernatant per clone
|
Antibody production & purification
|
• hybridoma culture
• ascites preparation
• Antibody purificaiton
|
2 week |
• 5-10 mg antibodies per clone
|
QC & subtype identification |
• SDS-PAGE
• antibody subtype verification
|
2 week |
• project report |