Rat monoclonal antibody|Rat monoclonal antibody service|Custom rat monoclonal antibody

Rat Monoclonal Antibody Service

Our Services

Rat monoclonal antibody can avoid immune tolerance caused by antigen homology of mouse protein. Rat McAb has PM affinity and higher specificity than mouse monoclonal antibodies. Dia-an's unique rat monoclonal antibody system, fuses activated rat B cells with tumor cells, and screens positive high specific rat monoclonal antibodies. Large-scale production of rat monoclonal antibody (ascites preparation or cell culture) can be obtained to meet the needs of scientific researchers or industrial customers.

Our advantages:

Rat monoclonal antibody can solve the problem of immune tolerance caused by antigen homology of mouse protein
Comprehensive antigen preparation platform
The prepared rat McAb has PM affinity and high specificity, better than mouse McAb
Ascites preparation or hybridoma cell supernatant culture can be selected to prepare purified antibody

Service Details

Steps	Specification	Timeline	Deliverables	Pricing
Immunogen Preparation	• Recombinant protein expression • Peptide synthesis and conjugation	1-4 weeks	• Design report • Protein sample • SDS-PAGE result	Quote (086)027-65522449 (086)027-59325199
Immunization and Titer test	• 4-8 rats immunization • Anti-serum titer ELISA test	8-10 weeks	• Anti-serum sample • ELISA titer
Fusion & Screening	• Hybridoma fusion • Positive clones screening • 2 rounds of subcloning	4-6 week	• 1-3 ELISA positive clones • 1-5 ml supernatant per clone
Antibody production & purification	• hybridoma culture • ascites preparation • Antibody purificaiton	2 week	• 5-10 mg antibodies per clone
QC & subtype identification	• SDS-PAGE • antibody subtype verification	2 week	• project report

Service Process

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Rat Monoclonal Antibody Service