Recently, the Institute of aquatic biology, Chinese Academy of Sciences Researcher Nie pin In the Journal of immunology, the team published a report entitled“ In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN- γ, IRF1, and Two Forms of CIITA ”This study describes IFN- γ The immune regulatory pathway mediated by the fish has existed in fish, and has obvious evolutionary conservative characteristics between fish and mammals.

Dr. Hou Jing is the first author of this paper, and researcher Nie pin is the corresponding author.

In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN- γ, IRF1, and Two Forms of CIITA

The Journal of Immunology

Publication date: March 18, 2020

Impact factor: 4.8

Sample: Zebrafish

Technical means: antibody preparation (Wuhan dai'an biological provides complete technical support)

▎ Background:

Scientists have found that in humans and mammals, IFN- γ– IRF1 – CIITA – MHCII regulatory pathway in interferon γ CIITA is the "molecular switch" of MHC class II gene expression. Human CIITA gene is regulated by four different promoter sequences, producing four different transcripts, which plays a key role in the regulation of MHC class II gene expression. The signal path mode is shown in the figure below.

Human signal pathway map (picture from paper)

▎ Research contents: 1

Researchers from the Institute of aquatic biology, Chinese Academy of Sciences, for the first time revealed the regulatory role of CIITA on MHCII in teleost zebrafish, and confirmed that in zebrafish, IFN- γ– IRF1 – CIITA – MHCII pathway is still conserved. However, two independent promoters P1 and P2 were found in CIITA of zebrafish, which control the two transcripts of CIITA respectively. A series of complex and rigorous experiments were conducted to verify the special mechanism of zebrafish IRF1 in this pathway. The signal path mode is shown in the figure below.

Signal pathway map of zebrafish

This study shows that IFN- γ The immune regulatory pathway mediated by the fish has existed in fish, and has obvious evolutionary conservative characteristics between fish and mammals.

There are three major protein antibodies in this paper IRF1, MHCII DAB, and CIITA protein They were prepared by Wuhan dai'an Biotechnology Co., Ltd.

The following is the research results of the preparation of antibody in this paper

Some research results (pictures from the paper)

Link to the original text:

http://www.jimmunol.org/content/early/2020/03/17/jimmunol.1801480

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