Products

FLAG-Tag Monoclonal Antibody

Cat No.: 2064
Specification:
Datasheet
  • Western Blotting of FLAG-GFP fusion protein expressed by HEK293 cells. Lane 1,2: dilution of 1:2500 Lane 3,4 : dilution of 1:5000 Lane 5,6 : dilution of 1:10000

  • Western Blotting of FLAG-tagged protein with 2064 at dilution of 1:5000

  • 293T cells transfected with the plasmid over- expressing 3×FLAG-CMV-10-FASN, using anti Flag antibody at 1:200 dilution

Product Name: FLAG-Tag Monoclonal Antibody
Cat No.: 2064
Clonality: Monoclonal
Species Reactivity: Flag-tagged Protein
Tested Applications: ELISA, WB, IP, IF, ChIP
Recommended Dilution: WB: 1:5000-10000; IP: 3ug/sample; IF: 1:100-200
Format: Liquid
Source: Mouse
Purification Method: Protein A/G Purified
Isotype: IgG2a
Conjugate: Unconjugated
Storage: Store at -20℃. Do not aliquot
Immunogen: Peptide DYKDDDDK
Full Name: FLAG-Tag
Synonyms: DYKDDDDK
Background: FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This antibody detects FLAG tag in most applications.
Category: Primary Ab
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