| Our Services |
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With enriched experience in bacterial protein expression， Dia-an offers a custom bacterial protein expression service for the efficient and cost-effective production of proteins. Our experience show that more than 90% proteins we conducted were soluable.
Our advantage:
- Evaluation report on gene design and codon optimization
- Expression and purification of 6xHis, TRX, GST, sumo, MBP and other tagged recombinant proteins
- Optimization of vector construction and optimization of induction conditions
- Subsequent processing, such asremoval, endotoxin removal, MS identification, biotin labeling, fluorescein labeling, animal immunity and other related services
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Steps
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Specification |
Timeline |
Deliverables |
Pricing |
| Gene synthesis |
• Gene synthesis and codon optimization
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1 week
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•Expression Vector
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Quote
(086)027-65522449
(086)027-59325199
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| Vector construction |
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
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1week
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| Expression evaluation |
• transformation into proper strain
• small-batch fermentation
•SDS-PAGE analysis to evaluate protein expression
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1week
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• Expression evaluation result
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Protein expression and purification（1mg）
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• fermentation based on expression evaluation
• protein purification
soluble expression-affinity purification
inclusion body expression-refolding
• SDS-PAGE and UV analysis
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2 weeks
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• Feasibility report
• If expression is feasible, provide a certain amount of protein sample to the client based on the expression. |
| Large-scale expression and purification (optional) |
• Flask or fermentation cylinder culture
• Affinity Purificaiton
• SDS-PAGE analysis
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Depend on scale
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• Purified protein
• SDS-PAGE report
• CoA |
| Our Promise |
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● Dia-an provide a variety of E. coli expression strains, supplemented by different expression conditions to ensure soluble expression.
● combined with gene design, codon optimization, protein expression and purification, the purity of prokaryotic protein expressed is higher than 90%.
● provide protein expression service report, with sequencing report, induction conditions, purification conditions and SDS-PAGE identification results.
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| Case Studies |
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Protein Expression Services
