DNA-RNA Hybrid Monoclonal Antibody |S9.6|DNA RNA Hybrid

Cat No.: 2112

Specification：

Immunofluorescentanalysis of ( 4% PFA) fixed HeLa cells using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500 and Alexa Fluor 647- conjugatedAffiniPure Goat Anti-Mouse IgG(H+L)
Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:500 and Alexa Fluor 647- conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
Chromatin immunoprecipitation analysis of HeLa cells genomic DNA(gDNA) using DNA-RNA hybrid Mouse Monoclonal Antibody [S9.6] at dilution of 1:200

Product Name:	DNA-RNA Hybrid Monoclonal Antibody [S9.6]
Cat No.:	2112
Clonality:	Monoclonal
Species Reactivity:	All Species
Tested Applications:	ELISA, IP, IF, CHIP, Dot Blot
Recommended Dilution:	IP: 1:50-500; IF: 1: 200
Format:	Liquid
Source:	Mouse
Purification Method:	Protein A/G Purified
Isotype:	IgG2a
Conjugate:	Unconjugated
Storage:	Store at -20℃. Do not aliquot
Immunogen:	ΦX174 bacteriophage-derived synthetic DNA/RNA
Full Name:	DNA-RNA Hybrid
Synonyms:	DNA-RNA hybrid; RNA/DNA hybrid
Background:	The DNA-RNA hybrids are a natural occurrence within eukaryotic cells and their level are high at sites of high transcriptional activity. They are non-canonical nucleic acid structures with transcriptional regulatory functions. Their presence is reported to predispose a locus to chromosomal breakage. The S9.6 monoclonal antibody recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA. The antibody has high affinity for DNARNA hybrids but also binds RNA-RNA hybrids that are AU-rich. The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since Rloop sequence affects binding affinity.
Category:	Primary Ab