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Service Details |
Steps
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Specification
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Timeline
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Pricing
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Immunization |
Antigen preparation |
Small molecule, peptide, recombinant protein, native protein can be used |
4 weeks |
QUOTE 027-65522449 027-59325199
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Immunization |
Host: alpaca;llama and Camel Naïve library can also be used for screening |
0-8 weeks | ||
Antibody Library Construction | cDNA synthesis | B cell sorting, RNA extraction and RT-PCR to get cDNA | 1 week | |
phagemid | PCR to amplify antibody gene and insert into phage vector | 4 weeks | ||
Library construction and identification |
The antibody gene fragments are amplified by PCR and connected to phage vector |
1week | ||
Identify the diversity and capacity of the library to ensure that the library capacity is greater than 108 and the correct insertion rate is greater than 90% | 2 weeks | |||
Antibody library |
Phage display antibody library is obtained by help phage rescue | 1 week | ||
Enriching and Screening | After 3-4 rounds of panning, antibodies with an affinity of more than 10-7m were obtained. | 3-4 weeks | ||
Verification of the positive clones by indirect ELISA |
1 week | |||
Delivery |
3-5 antibody sequences will be given according to standard protocol. The positive clone delivers 3. If you need more sequences, please contact us |
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Realted Service | Antibody expression | According to customer requirements, scFv,Fab, VHH can be expressed by prokaryotic expression system; intact IgG molecules can be expressed by CHO / 293 cells. | ||
Affinity assay |
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Epitope identification |
Case Studies |
Nanobody enroching and screening
Related Service |