Steps
|
Specification |
Timeline |
Deliverables |
Pricing |
Gene synthesis |
• Gene synthesis and codon optimization
|
1 week
|
•Expression Vector
|
Quote
(086)027-65522449
(086)027-59325199
|
Vector construction |
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
|
1 week
|
Expression evaluation |
• transfection into proper host cell(HEK293)
• small-scale transfection
•SDS-PAGE analysis to evaluate protein expression
|
1 week
|
• Expression evaluation result
|
Transient transfection and purification(30ml)
|
• transfection into suspension cultured cells
• protein purification
• SDS-PAGE and ELISA analysis
|
2 weeks
|
• Feasibility report
• If expression is feasible, provide a certain amount of protein sample to the client based on the expression. |
Stable cell line construction
|
• stable transfect and screen
• identification(qPCR & FACS)
• Subcloning
• identification(qPCR & FACS)
• stable cell line pool
|
10 weeks
|
• 2-3 stable cell line
• report
|
Large-scale expression and purification (optional) |
• 1L Flask or fermentation cylinder culture
• Affinity Purificaiton
• SDS-PAGE analysis
|
Depend on scale
|
• Purified protein
• SDS-PAGE report
• CoA |